CERATIS – glycerinated solution intended for use in the ear cavity

Symposium: Medication – From Concept to Use – 2nd Edition, Iași, May 20–22, 2009
Authors: Ana Ofrim, Marinela Panait

Abstract:
CERATIS is presented as an ear solution product designed for use in the ear cavity. The product cleans and protects the ear mucosa and maintains its normal functionality. It is a glycerinated solution preparation with moisturizing, emollient, and antiseptic properties, supporting the regeneration and healing of the tissues of the external auditory canal.

RINOTISOL – natural product in the form of nasal drops

Symposium: Medication – From Concept to Use – 2nd Edition, Iași, May 20–22, 2009
Authors: Ana Ofrim, Marinela Panait

Abstract:
Pharmacotoxicological and clinical data obtained from studies regarding the use of a preparation with a high content of water-soluble propolis components (polyphenols) and multiple bioactive properties are presented. The liquid propolis extract, a bioactive intermediate of the apitherapeutic product RINOTISOL, was developed using a non-destructive technology that preserves the main chemical compounds with therapeutic effectiveness. The characterization and quantitative analysis of the bioactive compounds were performed using spectrophotometric and chromatographic methods.

TISAICIN – natural product containing extracts from Capsicum annum, Salix alba, Arctium lappa, and menthol

Symposium: Medication – From Concept to Use – 2nd Edition, Iași, May 20–22, 2009
Authors: Ana Ofrim, Marinela Panait

Abstract:
Developed as a result of proprietary research, TISAICIN is a natural cream product that stimulates and activates blood circulation in the area of application and has analgesic properties for muscular and joint pain.

Identification of “polyphenol” bioactive compounds through HPLC from various plant extracts

Symposium: Medication – From Concept to Use – 2nd Edition, Iași, May 20–22, 2009
Authors: Monica Popescu, Nicoleta Mărgărit, Maria Alexandra Țapu

Abstract:
The paper presents the chromatographic fingerprints of several plant extracts produced by S.C. TIS Farmaceutic, used as bioactive intermediates in the company’s own products.

Obtaining carotenoid concentrates: extraction, separation, purification, and stabilization technology

Symposium: Medication – From Concept to Use – 2nd Edition, Iași, May 20–22, 2009
Authors: Monica Popescu, Nicoleta Mărgărit, Maria Alexandra Țapu

Abstract:
The paper presents experimental studies conducted on two plant sources (Capsicum annum and Hippophae rhamnoides) in order to establish the extraction, purification, and standardization technology for carotene concentrates.

Monitoring the stability of products developed by TIS Farmaceutic

Symposium: Medication – From Concept to Use – 2nd Edition, Iași, May 20–22, 2009
Authors: Ioana Oprea, Daniela Popa, Monica Popescu, Nicoleta Mărgărit

Abstract:
The paper presents the results obtained from microbiological and physicochemical analyses carried out throughout the shelf life of products developed by TIS Farmaceutic.

Experimental studies regarding the antimicrobial properties of natural extracts

Symposium: Medication – From Concept to Use – 2nd Edition, Iași, May 20–22, 2009
Authors: Daniela Popa, Ioana Oprea

Abstract:
The paper presents experimental studies conducted with several types of natural extracts, focusing on their ability to inhibit the development of specific microorganisms.

Identification and quantitative determination of capsaicin from Capsicum annum, intermediates, and finished products

Symposium Medication – From Concept to Use – Iași, May 30–June 1, 2007

Authors:
Monica Popescu, Cătălina Niculae (TIS Farmaceutic SA, Bucharest)

Abstract:
The paper presents the experimental study carried out to establish the method for quantitative determination of capsaicin and its identification through UV-VIS spectrophotometry.

APICRISIN-D propolis-based medication

Symposium Medication – From Concept to Use – Iași, May 30–June 1, 2007

Authors:
Ana Ofrim, Monica Popescu (TIS Farmaceutic SA, Bucharest)

Abstract:
The bioactive complex from propolis, obtained through a standardized technology, forms the basis of the medication APICRISIN-D, a topical solution recommended for the treatment of shingles.
This paper presents the data obtained to support this natural medication, with antiviral, anti-inflammatory, and mildly analgesic action, enabling its therapeutic use in the treatment of various clinical forms of infections with cutaneous and mucosal localization caused by Herpes simplex virus (HSV) types 1 and 2 and Varicella Zoster virus (VZV), in both adults and children.

Quantitative determination of ciprofloxacin hydrochloride and fluocinolone acetonide from the medication CIPROTIS®. Validation of the analytical method

Symposium Medication – From Concept to Use – Iași, May 30–June 1, 2007

Authors:
Marinela Panait, Catalina Niculae (TIS Farmaceutic SA, Bucharest)

Abstract:
For the quantitative determination of the two active substances (ciprofloxacin hydrochloride and fluocinolone acetonide) in the medication CIPROTIS®, an HPLC analytical method allowing their simultaneous determination was developed and validated. The validation studies of the analytical method are presented.

Bioactive extract from Capsicum annum, possible use as a preservative agent

Symposium Medication – From Concept to Use – Iași, May 30–June 1, 2007

Authors:
Daniela Gheorghe, Cătălina Niculae (TIS Farmaceutic SA, Bucharest)

Abstract:
The purpose of this paper is to determine whether Capsicum annum extracts (propylene glycolic and oleoresin extracts) can be used as preservative agents in various pharmaceutical and cosmetic formulations.

KETO-TIS, foaming hair gel original medication, combination of propolis and ketoconazole

Symposium Medication – From Concept to Use – Iași, May 30–June 1, 2007

Authors:
Ana Ofrim, Monica Popescu (TIS Farmaceutic SA, Bucharest)

Abstract:
The paper presents the complete experimental studies carried out with the product “KETO-TIS, foaming hair gel” in order to establish a pharmaceutical formula with therapeutic efficiency intended for the treatment of seborrheic dermatitis and combating the proliferation of the microorganism Pityrosporum ovale, which causes dandruff formation.

Experimental study regarding the demonstration of the “preservative” capacity of propolis extracts

Symposium Medication – From Concept to Use – Iași, May 30–June 1, 2007

Authors:
Daniela Gheorghe, Ana Ofrim (TIS Farmaceutic SA, Bucharest)

Abstract:
Experimental studies regarding the antimicrobial action of certain types of bioactive propolis extracts, also used in product formulations, are presented.
The objective was to demonstrate the possible antimicrobial effects of two types of propolis extracts (aqueous propolis extract and propylene glycol propolis extract) and their subsequent use in product formulations (medicines, cosmetic products, and nutritional supplements). Based on the obtained results, studies with the natural extracts used in product formulations will be resumed. Depending on the results obtained, the study of the antimicrobial effectiveness of natural extracts will continue, both for each type of extract and for combinations of natural extracts.

Preliminary study regarding the chromatographic (HPLC) quantification of polyphenolcarboxylic acids from plantain and derived extracts

National Conference on Phytotherapy, 4th Edition – Iași, May 14–16, 2008
Authors: Monica Popescu, Catalina Niculae, Nicoleta Margarit (TIS Farmaceutic SA, Bucharest)
Abstract:
The paper presents preliminary studies carried out in order to establish a chromatographic analysis method (HPLC) for polyphenolcarboxylic acids from plantain and derived extracts.

Simultaneous quantitative determination by HPLC method of diclofenac sodium and methyl paraben from the drug Diclofenac-T

National Conference on Phytotherapy, 4th Edition – Iași, May 14–16, 2008
Authors: Catalina Niculae (TIS Farmaceutic SA, Bucharest)
Abstract:

The experimental quantitative method for the simultaneous determination by high-performance liquid chromatography of diclofenac sodium and methyl paraben from the gel-form medication Diclofenac-T was established.

HPLC identification of flavonoids (chrysin) from propolis extract obtained from various raw material sources

National Conference on Phytotherapy, 4th Edition – Iași, May 14–16, 2008
Authors: Monica Popescu, Catalina Niculae, Nicoleta Margarit (TIS Farmaceutic SA, Bucharest)
Abstract:
Experimental laboratory studies enabled the development of a high-performance chromatography identification method for flavonoids from various propolis sources and derived extracts.

Apiphytotherapeutic products developed through applied experimental research at SC TIS Farmaceutic SA

National Conference on Phytotherapy, 4th Edition – Iași, May 14–16, 2008
Authors: Ana Ofrim, Monica Popescu, Daniela Popa, Catalina Niculae (TIS Farmaceutic SA, Bucharest)
Abstract:
The research and development strategy for the apiphytotherapeutic product range (medicines, cosmetic products, and nutritional supplements) focused on studying the production of bioactive extracts from medicinal plants and propolis.
Several medicinal plants were studied as potential sources of bioactive intermediates usable in pharmaceutical and cosmetic formulas; to date, standardized bioactive extracts from 20 plants have been developed.
Depending on their intended use, the extracts were obtained using proprietary technologies adapted to the proposed purpose.
So far, three types of extracts have been obtained: hydroalcoholic extracts (using ethyl alcohol in various concentrations), concentrated propylene glycol extracts, and aqueous extracts.

Identification and quantification of active principles (polyphenolcarboxylic acids) from Plantago species, extracts, and products

National Symposium of Pharmacy, 2nd Edition – Timișoara, September 24–26, 2008
Authors: Monica Popescu, Nicoleta Mărgărit, Cătălina Niculae
Abstract:
The paper presents experimental studies conducted to establish a high-performance chromatographic analysis method for polyphenolcarboxylic compounds from plantain, derived extracts, and products.

Experimental study regarding the microbiological stability of phytotherapeutic products containing plantain extracts

National Symposium of Pharmacy, 2nd Edition – Timișoara, September 24–26, 2008
Authors: Daniela Popa, Monica Popescu, Cătălina Niculae, Nicoleta Mărgărit

Abstract:
The study presents the results of microbiological analyses obtained from stability studies of two types of plantain extracts, as well as products containing these extracts (medicines, nutritional supplements, cosmetic products).

Development of a new anti-inflammatory topical medication based on diclofenac

National Symposium of Pharmacy, 2nd Edition – Timișoara, September 24–26, 2008
Authors: Ana Ofrim, Marinela Panait, Cătălina Niculae, Daniela Popa
Abstract:
The paper presents the development of a new anti-inflammatory medication based on the association of a nonsteroidal anti-inflammatory drug (NSAID) and a Capsicum annum extract with revulsive effects.

Bioactive propolis extracts usable in various formulations

National Symposium of Pharmacy, 2nd Edition – Timișoara, September 24–26, 2008
Authors: Monica Popescu, Cătălina Niculae, Nicoleta Mărgărit
Abstract:
Experimental studies describe the development of a high-performance chromatographic method for identifying flavonoids and polyphenolcarboxylic acids from various propolis sources, derived extracts, and different formulations (nutritional supplements, medical devices).

Preliminary experimental studies carried out for the development of the new “Calendula Gel” preparation with pharmacodynamic action

National Conference on Phytotherapy, 4th Edition – Iași, May 14–16, 2008
Authors: Ana Ofrim, Monica Popescu, Cătălina Niculae, Daniela Popa, Nicoleta Mărgărit
Abstract:
Experimental research focused on establishing an analytical protocol for the quantitative and qualitative characterization of active principles from Calendula officinalis, intermediate extracts, and finished products; accurate dosing of active principles with distinct pharmacodynamic action was pursued.

FLAVOSOL-T, 30% propolis tincture – medication with multiple therapeutic properties

DRUG RESEARCH BETWEEN INFORMATION AND LIFE SCIENCES – 4th Edition, Bucharest, October 19–20, 2006

Authors:
Ana Ofrim, Monica Popescu, Mariana Borş (TIS Farmaceutic SA, Bucharest)

Abstract:
The paper presents the extraction, separation, and processing technology of the product FLAVOSOL-T, 30% propolis tincture, as well as the standardization of the propolis tincture starting from the raw material – propolis.

Preliminary experimental tests regarding the possibility of replacing preservatives with natural extracts in cosmetic products

DRUG RESEARCH BETWEEN INFORMATION AND LIFE SCIENCES – 4th Edition, Bucharest, October 19–20, 2006

Authors:
Daniela Gheorghe, Ana Ofrim, Monica Popescu (TIS Farmaceutic SA, Bucharest)

Abstract:
Within the preliminary study, a combination of natural extracts with potential preservative roles was proposed for later use in the formulation of cosmetic products.
The composition of the tested “natural preservative” that could be used in a topical-use solution was as follows:
– 30% aqueous propolis extract
– 2.5% propylene glycol willow extract
– 2.5% propylene glycol arnica extract
Preliminary experimental data allow us to anticipate the use of natural extracts as preservatives in TIS Farmaceutic products. The extracts used are obtained in the company’s own facility of SC TIS Farmaceutic SA, authorized by the National Medicines Agency. Natural extracts are used in medicinal products, cosmetic products, and nutritional supplements alike.

Identification and quantitative determination of carotenoids in different plant material sources

DRUG RESEARCH BETWEEN INFORMATION AND LIFE SCIENCES – 4th Edition, Bucharest, October 19–20, 2006

Authors:
Ana Ofrim, Monica Popescu, Catalina Niculae (TIS Farmaceutic SA, Bucharest)

Abstract:
A quantitative method for carotenoid determination was established through the quantitative analysis of β-carotenes from sea buckthorn, calendula, and Capsicum annum, and their identification by UV-VIS molecular absorption spectrophotometry.

Plantaginol, plantain syrup – natural medication

National Conference on Phytotherapy, 3rd Edition – Iași, May 28–31, 2006 and the 4th International Conference of the Association for Medicinal and Aromatic Plants of South-East European Countries

Authors: Ana Ofrim, Monica Popescu, Mariana Borş, Daniela Gheorghe (TIS Farmaceutic SA, Bucharest)

Abstract:
The phytotherapeutic product Plantaginol syrup (20% plantain extract) has soothing, emollient, and decongestant properties on irritated respiratory tract mucosa and is indicated in various bronchopulmonary conditions such as tracheobronchitis, acute or chronic bronchitis associated with irritative cough.

Preliminary study regarding the use of plant extracts as natural preservatives

National Conference on Phytotherapy, 3rd Edition – Iași, May 28–31, 2006 and the 4th International Conference of the Association for Medicinal and Aromatic Plants of South-East European Countries

Authors:
Daniela Gheorghe, Ana Ofrim, Monica Popescu (TIS Farmaceutic SA, Bucharest)

Abstract:
Within the preliminary study, the contamination of certain nutritional supplements and plant extracts with aerobic microorganisms was monitored at different time intervals. The determinations were necessary to observe whether there were significant differences between products containing synthetic preservatives and those without preservatives. In the case of plant extracts, contamination levels were also analyzed over different periods of time.
The study of the preservative properties of plant extracts will involve monitoring their effects on typical microorganisms commonly contaminating products, such as: Candida albicans, Aspergillus niger, Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus.
Preliminary experimental data allow us to anticipate the use of natural extracts as preservatives in TIS Farmaceutic products. The study is still in its preliminary phase, and based on the obtained results, the minimum inhibitory concentration (MIC) will be established for each tested microorganism (Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Candida albicans, Aspergillus niger) and for each type of extract.

Plantain Between Empiricism and Scientific Rigor

National Conference on Phytotherapy, 3rd Edition – Iași, May 28–31, 2006 and the 4th International Conference of the Association for Medicinal and Aromatic Plants of South-East European Countries

Authors:
Ana Ofrim, Monica Popescu (TIS Farmaceutic SA, Bucharest)

Abstract:
Plantain, an exceptionally valuable plant product, is a natural “reservoir” of bioactive compounds with important specific pharmacodynamic actions: healing, epithelial regenerative, restorative, and bacteriostatic.

The propylene glycol concentrated extract of plantain, obtained through a non-destructive technology, contains a high concentration of polyphenolic compounds, polysaccharides, simple sugars, and flavonoids, which provide the product with healing effects, support the regeneration of dermal tissue affected by burns and wounds of various etiologies (varicose ulcers, leg ulcers, atonic ulcers), and help stop local infections.

The principle responsible for the pharmacological action of the phytotherapeutic product, the concentrated propylene glycol extract of plantain, was selected following numerous pharmacological and clinical studies that demonstrated its therapeutic actions: healing, restorative, bacteriostatic, and anti-inflammatory, as well as indications presented in specialized literature.

The choice of the pharmaceutical form, a hydrogel for the PlantagoTIS product containing plantain extract in propylene glycol as the active principle, was made following the review of recent scientific data based on extensive phytotherapy research demonstrating the effectiveness of medicines derived from plant products. The pharmacodynamic action of phytotherapeutic products is mainly due to the active substances, but also to the synergistic or potentiating effects generated by other components present in smaller quantities within the plant extract.

Plantain: Between Empiricism and Scientific Rigor

3rd National Conference of Phytotherapy – Iaşi, May 28-31, 2006, and the 4th International Conference of the Association for Medicinal and Aromatic Plants of Southeast European Countries

Authors:
Ana Ofrim, Monica Popescu (TIS Farmaceutic SA, Bucharest)

Abstract:
Plantain, an exceptionally valuable herbal product, is a natural "reservoir" of bioactive compounds with significant specific pharmacodynamic actions: healing, epithelializing, reconstitutive, and bacteriostatic.

The concentrated plantain extract in propylene glycol, produced through non-destructive technology, features a high concentration of polyphenolic compounds, polysaccharides, simple sugars, and flavonoids. These provide the product with healing effects, restoration of dermal tissue affected by burns or wounds of various etiologies (varicose ulcers, leg ulcers, atonic ulcers), and the ability to halt local infections.

The principle responsible for the pharmacological action of the phytotherapeutic product—the concentrated plantain extract in propylene glycol—was selected following numerous pharmacological and clinical studies that highlighted its therapeutic actions: healing, reconstitutive, bacteriostatic, and anti-inflammatory, as well as indications present in specialized literature.

The choice of the pharmaceutical form, a hydrogel for the PlantagoTIS product containing plantain extract in propylene glycol as the active ingredient, was made following consultations of recent scientific data. Persistent research in phytotherapy has demonstrated the efficacy of medicines derived from herbal products. The pharmacodynamic action of phytotherapeutic products is primarily due to the active substances, but also to the synergism or potentiation determined by other components found in smaller quantities within the herbal extract.

Qualitative and Quantitative Physico-Chemical Analysis of Propolis from Different Geographical Regions. Validation of Analytical Methods

Authors:
Ctin. Luca (1), Silvia Andrei (2), Marinela Panait (2), Monica Popescu (2)
(1) Gheorghe Asachi Technical University of Iaşi, Faculty of Industrial Chemistry
(2) TIS Farmaceutic S.A. Bucharest